Baseclick’s PCR-Click labeling kits contains all reagents required for generating highly labeled PCR products via an esy-to-handle two steps method based on click chemistry. In the triphosphate mixtures dTTP is replaced by C8-Alkyne-dUTP or dCTP is replaced by C8-Alkyne-dCTP. Both mixtures can be used in standard PCR reactions with the optimized Baseclick Polymerase. The resulting PCR products contain alkyne moieties, which react with azido-derivates of fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. The label incorporation can be tuned by the amount of marker azide used in the click reaction. Labeling rates of up to 20 % or more can be achieved!
Labeling of PCR fragments: