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Product Name:
RNA Labeling Kit - 647

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For research use only!

Text:

Baseclick’s RNA Labeling Kit has been developed for the production of alkyne-labeled RNA targets by in vitro transcription from bacteriophage T7 RNA polymerase promoters. Since multiple rounds of transcription can happen on a single DNA template, many RNA transcripts of the template DNA are produced. The kit utilizes 5-ethynyl-UTP (5-EUTP) to label the RNA with alkynes. The resulting RNA contains alkyne modified uracils, which react with azido-derivates of fluorescent dyes, haptenes and other labels in a highly selective fashion under benign click reaction conditions. The label incorporation can be tuned by the amount of marker azide used in the click reaction.

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