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PCR Labeling Kits

Baseclick’s PCR-Click labeling kits contain all reagents required for generating highly labeled PCR products via an easy-to-handle two steps method based on click chemistry. In the triphosphate mixtures dTTP is replaced by C8-Alkyne-dUTP or dCTP is replaced by C8-Alkyne-dCTP. Both mixtures can be used in standard PCR reactions with the optimized Baseclick Polymerase. The resulting PCR products contain alkyne moieties, which react withazido-derivates of fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. The label incorporation can be tuned by the amount of marker azide used in the click reaction. Labeling rates of up to 20 % can be achieved!

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