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Product Name:
EdU DetectPro FC Kit 647

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For research use only!

Text:


The new and special DetectPro dye system within the detection procedure allows for higher sensitivity as well as for a better signal-to-noise ratio by significantly diminishing the background without altering the outstanding fluorescence levels.

Kit contents:

 Vial-label  Amount  Component Component long term storage Kit short term storage *
Component A
yellow
 10 mg 5-Ethynyl-deoxyuridine (5-EdU)  -20°C  2 - 8°C
 
 Dark
 
 Do not freeze
 
 Dry
Component B
red
 60 µL Eterneon2 GREEN Azide (BCK-EdUPro-FC488)
Eterneon2 RED Azide (BCK-EdUPro-FC647)
 -20°C
 dark
Component C
violet
 5 mL DMSO  RT
Component D
green
 2 mL Reactor system  2 – 8°C
Component E  50 mL Saponin-based reagent (10x solution)  2 – 8°C
Component F
 
 5 mL Fixation solution (4% Paraformaldehyde)  2 – 8°C
Component G
blue
 400 mg Buffer additive  -20°C**
Component H
orange
 2 x 2 mL Reaction buffer (10x)  2 – 8°C
* This kit is stable up to 1 year after receipt, when stored as directed.
** When dissolved the component E has to be kept at -20°C for long-term storage. Prepare aliquots to avoid too many freeze and thaw cycles; if the solution starts to develop a brown colour, it has degraded and should be discarded

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