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Product Name:
EdU-Click 647

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The detection of cell proliferation is of utmost importance for monitoring of cell vitality, determining genotoxicity or evaluating anticancer drugs. The baseclick EdU (5-ethynyl-2’-deoxyuridine) cell proliferation assays provide a superior alternative to bromodeoxyuridine (BrdU) assays. Baseclick’s EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU-Click Assay utilizes click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol both reduces the total number of steps and significantly decreases the total amount of time.

Kit contents

Vial-label Amount Component Component long term storage Kit storage
yellow 5 mg 5-Ethynyl-deoxyuridine (5-EdU) -20°C 2 - 8°C
Do not freeze
red 130 µL Eterneon-Red 645 Azide
-20°C, dark
purple 2 x 2 mL DMSO RT
orange 4 x 2 mL Reaction buffer (10x) 2 – 8°C
green 2 x 2 mL Catalyst solution RT
blue 4 x 200 mg Buffer additive -20°C