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The detection of cell proliferation is of utmost importance for monitoring of cell vitality, determining genotoxicity or evaluating anticancer drugs. The baseclick EdU (5-ethynyl-2’-deoxyuridine) cell proliferation assays provide a superior alternative to bromodeoxyuridine (BrdU) assays. Baseclick’s EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU-Click Assay utilizes click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol both reduces the total number of steps and significantly decreases the total amount of time.