Cell Proliferation Kits
The baseclick EdU-Click Assays overcome these limitations, providing a superior alternative to BrdU and [3H]thymidine assays for measuring cell proliferation. EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside.
The detection of cell proliferation is of utmost importance for assassing cell health, determining genotoxicity or evaluating anticancer drugs. This is normally performed by adding nucleoside analogs like [3H]thymidine or 5-bromo-2’-deoxyuridine (BrdU) to cells during replication, and their incorporation into DNA is detected or visualized by autoradiography or with an anti-BrdU-antibody respectively. Both methods exhibit several limitations. Working with [3H]thymidine is troublesome because of its radioactivity. Autoradiography is slow and thus not suitable for rapid high-throughput studies. The major disadvantage of BrdU staining is that the double-stranded DNA blocks the the access of the anti-BrdU antibody to BrdU units. Therefore samples have to be subjected to harsh denaturing conditions resulting in degradation of the structure of the specimen.