With the emergence of click chemistry the use of azide and alkyne derivatised α amino acids has found high interest e.g. in the area of peptide synthesis as a powerful tool in the development of new therapeutics and biological chemistry fundamental research.
As peptide labelling mostly requires reactions which are close to physiological conditions (neutral pH, aqueous solution, ambient temperature) Cu (I) catalyzed Alkyne- Azide (CuAAC) Click reaction is optimal for such biological compounds. The advantages of the click chemistry once again show here their potential. The low reactant concentrations, low background labelling, the quantitative and rapid labeling, plus the mild reaction conditions fit the necessary reactivity, selectivity and biocompatibility criteria for the nucleic acid labelling and thus ensure the preservation of biological function.
Base click provides a high quality azido amino acids. In this form they can easily undergo CuAAC click reaction when a alkyne functional reporter molecule is present.