IVT High Performance T7 RNA Synthesis Kit
High performance RNA synthesis kit
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Size | Catalog No. | Price |
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1 Kit / 50 Assays | BCK-RNA-IVT50 | € 230,00 |
1 Kit Plus / 50 Assays | BCK-RNA-IVT50+ | € 290,00 |
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Our IVT high performance RNA synthesis kit offers an efficient, high-performance solution for in vitro mRNA production. Designed for researchers and biotech professionals, this kit provides all the essential components needed to generate high-quality mRNA for use in various applications, including vaccine development and therapeutic mRNA production.
Kit Components
• T7 RNA Polymerase (GMP-grade): High-performance enzyme for in vitro transcription, ensuring efficient synthesis of mRNA.
• Pyrophosphatase (GMP-grade): Drives the transcription reaction by hydrolyzing pyrophosphate, improving RNA yield.
• 5x Transcription Buffer: Optimized for maximum reaction efficiency.
• CTP, UTP, GTP, ATP (100 mM each): High-purity triphosphates for precise RNA incorporation.
• Linear DNA Control Template: Ensures quality control and consistency in transcription performance.The IVT high performance RNA synthesis kit includes GMP-grade T7 RNA polymerase and pyrophosphatase, which are essential for ensuring high-yield and efficient transcription. The inclusion of pyrophosphatase helps shift the equilibrium of the transcription reaction, optimizing mRNA production. This feature makes the IVT high performance RNA synthesis kit an ideal choice for those who require reliable and reproducible results.
Our IVT Kit for mRNA synthesis is designed to support your research and development efforts with top-tier reagents and enzymes. The kit includes a 5x transcription buffer, CTP, UTP, GTP, ATP (all 100 mM), and a linear DNA control template (mScarlet) for quality assurance. These components work together to guarantee the success of your mRNA synthesis process, making our kit a powerful tool for your laboratory.
Key Features
• Unmatched Quality: GMP-grade enzymes manufactured with stringent quality control standards.
• High Efficiency: Optimized for maximum RNA yield and accuracy.
• Flexible Applications: Ideal for mRNA research, preclinical studies, and the incorporation of modified nucleotides.FAQ
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What is the difference between IVT High Performance T7 RNA Synthesis Kit and the Plus version?
IVT High Performance T7 RNA Synthesis Kit Plus includes DNase I and RNase inhibitor for cleaner and more efficient mRNA production.
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Can this kit be used for therapeutic applications?
Yes, the GMP-grade enzymes and reagents make it suitable for therapeutic research and preclinical applications.
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Are modified nucleotides included in the kit?
Modified nucleotides are not included in the kit but can be purchased from us.
See our Products – Nucleotides section. We offer:
- Stabilizing triphosphates for RNA
- Triphosphate for modifying of 3’END RNA
- Ethynyl-UTP for longer protein expression [1]
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How can I improve a low yield of RNA from the transcription reaction?
If the transcription reaction with your template produces full-length RNA, but the yield is significantly lower than expected, it is possible that contaminants in the DNA template are inhibiting the T7 RNA polymerase, or the DNA concentration is incorrect.
Therefore, additional purification of the DNA template is recommended.
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What is the difference between IVT High Performance T7 RNA Synthesis Kit and the Plus version?
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Shelf Life
12 months unopened after receipt
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Storage Conditions
– 20 °C
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Physical State
kit system made of different components
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CAS Number
n.a.
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Preparation/Handling
please see user manual of the kit
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Shelf Life