Fluorescein Azide (6-FAM-Azide)

Fluorescein Azide (6-FAM-Azide) is a fluorescent dye that allows efficient labeling of biomolecules by click reactions. With identical spectral properties to FITC (Fluorescein-5,6-isothiocyanate), ATTO488, Alexa Fluor 488 or DyLight 488 and alike, it is well suited for most fluorescent detection methods.

LITERATURE

Postsynthetic DNA Modification through the Copper-Catalyzed Azide–Alkyne Cycloaddition Reaction, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 8350–8358.

https://doi.org/10.1002/anie.200802077

Click–Click–Click: Single to Triple Modification of DNA, P. M. E. Gramlich et al., 2008, Angew. Chemie Int. Ed., Vol. 47, p. 3442-3444.

https://doi.org/10.1002/anie.200705664

Synthesis of hemicyanine dyes for ‘click’ bioconjugation, W. H. Zhan et al., 2005, Tetrahedron Lett., Vol. 46, p. 1691–1695.

https://doi.org/10.1016/j.tetlet.2005.01.066

DNA visualization in single molecule studies carried out with optical tweezers: Covalent versus non-covalent attachment of fluorophores, S. Suei et al., 2015, Biochemical and Biophysical Research Communications, Vol. 466(2), p. 226-231.

https://doi.org/10.1016/j.bbrc.2015.09.013

Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis, L. Aliyeva-Schnorr et al., 2016, Chromosome Research, Vol. 24, p. 231–242.

https://doi.org/10.1007/s10577-016-9518-8

Cohesin depleted cells rebuild functional nuclear compartments after endomitosis, M. Cremer et al., 2020, Nature Communications, Vol. 11, p. 6146.

https://doi.org/10.5061/dryad.vt4b8gtqb