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Product Name:
EdU Flow Cytometry Kit 647 for 100 assays

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For research use only!

Text:

Description

The baseclick EdU Flow Cytometry Kits provide a superior alternative to BrdU and [3H]thymidine assays for directly measuring DNA synthesis. EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU Flow Cytometry Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Flow Cytometry Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Kit contents

Component amount storage
5-Ethynyl-2'-deoxyuridine (EdU) 20 mg -20 °C
Eterneon Red 645 Azide 2 x 130 μL (10 mM in DMSO) -20 °C, dark
DMSO 8.5 mL RT
Fixative Solution 2 x 5 mL 2-8 °C
Permeabilization and wash reagent 2 x 50 mL 2-8 °C
Catalyst solution 2 mL RT
Buffer additive 400 mg -20 °C
 


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