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Baseclick’s PCR-Click 488 labeling kit contains all reagents required for generating highly labeled PCR products via an easy-to-handle two steps method based on click chemistry. This kit is used for the synthesis and labeling of alkyne-modified DNA via PCR and copper catalyzed click reaction. The labeling of alkyne-modified PCR fragments with 6-FAM Azide via copper-catalyzed azide-alkyne cycloaddition (CuAAC; Click Chemistry).Labeling rates (% of fluorescently tagged bases) of the labeled PCR product can be adjusted by the amount of marker azide 06-FAM Azide, used in the click reaction. Labeling rates of up to 20 % and even more can be achieved.
Kit contents :